Energia na Agricultura, Botucatu, v. 38, n. 4, p. 69-79, outubro-dezembro, 2023 69
ISSN 2359-6562 (ONLINE) 2359-6562 (CD- ROM)
PROTOCOLO DE PRODUÇÃO DA FARINHA DE ESTIPE DO COGUMELO SHIITAKE JÚLIA LIMA NUNES1 E ROGÉRIO LOPES VIEITES 2
1Engenheira de alimentos pela UFSCar e mestre pelo curso pós graduação na Energia na Agricultura da Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP)/Faculdade de Ciências Agronômicas (FCA) – Campus Botucatu. Rodovia Alcides Soares, Km 3 – Fazenda Experimental Lageado - Botucatu/SP - CEP 18610-034. E- mail: julia.l.nunes@unesp.br.
2Professor Titular Departamento de Produção Vegetal/Horticultura da Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP)/Faculdade de Ciências Agronômicas (FCA) – Campus Botucatu. Rodovia Alcides Soares, Km 3 - Fazenda Experimental Lageado - Botucatu/SP - CEP 18610-034. Email: rogerio.vieites@unesp.br.
RESUMO: O objetivo deste trabalho foi realizar o protocolo de produção da farinha de estipe de cogumelo shiitake (Lentinula edodes), de forma a aproveitá-lo e tornar possível o subproduto na alimentação humana. Para o desenvolvimento da farinha de estipe, o processo seguiu com as seguintes etapas: congelamento lento dos talos de shiitake; descongelamento; higienização; resfriamento; branqueamento; corte; secagem; acondicionamento; armazenamento; moagem e peneiragem. Os resultados obtidos nas análises foram: umidade (5,62%); L* ( 31,77); ºHue (45º); Chroma (0,55); pH (5,90) e Aw (0,44). Por fim, no presente estudo, pode-se concluir que a farinha de estipe de cogumelo shitake apresentou baixa atividade de água, mostrando ser um produto com estabilidade microbiológica, ligeiramente escura de acordo com seu teor de luminosidade, baixo teor de umidade e pode ser recomendada na alimentação.
Palavras chaves: píleo; processamento; produção.
PROTOCOL FOR PRODUCING SHIITAKE (Lentinula edodes) STYLE FLOUR
ABSTRACT: The objective of this work was to develop a production protocol for the flour of the shiitake mushroom Lentinula edodes to reuse it and make it a possible byproduct in human food. For the development of the shiitake flour, the process involved the following steps: slow freezing of the shiitake stalks; thawing; hygiene; cooling; blanching; cutting; drying; packaging; storage; and grinding and sieving. The results obtained in the analyses were as follows: humidity (5.62%); L* (31.77); °hue (45°); chroma (0.55); pH (5.90) and Aw (0.44). Finally, in the present study, the flour from the shiitake mushroom stipe flour presented low water activity, indicating that the flour is a product with microbiological stability, is slightly darker in terms of luminosity, has low moisture content and can be recommended for use in food.
Keywords: pileus; processing; production.
1 INTRODUCTION
Mushrooms, thousands of years ago, began to be appreciated in Eastern and European cuisine because of their high nutritional value and medicinal treatments. Its global production has increased in recent years, reaching just over 10 million tons in 2017, five times greater than that produced in 1990 (Faostat, 2017). In Brazil, an increase in mushroom production has also been
observed due to the current growing demand from the population and the popularization and growth of oriental restaurants in the country (Associação Nacional dos Produtores de Gugumelos, 2013).
Currently, the Ministry of Industry, Foreign Trade and Services (2023) is working on calculating national production data. After a hiatus in the survey during the pandemic years, projections for the year 2023 pointed to
Recebido em 05/09/2023 e aprovado para publicação em 11/09/2023 DOI: http://dx.doi.org/10.17224/EnergAgric.2023v38n4p69- 79
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production of up to 14 thousand tons. The dollar value of mushroom and truffle production in 2019 was 44,653.506; in 2020, it was 43,774.112; in 2021, it was 500,445.74; and in 2022, it was 465,148.03 (Faostat, 2024).
The agro-industrial branch aimed at mushroom production is called fungiculture, a species of fungi also known as macrofungi (Martínez-Ibarra; Gómez-Martín; Armesto - López, 2019). Among the known mushrooms or fungi that produce fruiting bodies, 50% are edible, 18% are medicinal, 10% are poisonous , and 22% still have properties of commercial interest; however, these properties have not yet been established (Floudas et al., 2012).
Shiitake cultivation can be divided into two types: rudimentary, which is carried out on wooden logs, and synthetic, which is carried out on synthetic substrates. In the form of logs, which are currently the most common, cultivation is carried out on eucalyptus wood, and synthetic substrates are made from agricultural residues and other types of waste, such as rice bran, wood dust, wheat bran, corn straw, gypsum, lime, and limestone (Urben, 2017).
The large mushroom producers in Brazil are concentrated in the state of São Paulo, where approximately 500 producers generate R$21 million reais, but production also occurs in the states of Minas Gerais, Paraná, Rio de Janeiro, Brasília, Rio Grande do Sul, Bahia and Pernambuco. Among the producers in the state of São Paulo, the cities that stand out the most are Mogi das Cruzes, Pinhalzinho, Ibiúna, Sorocaba, Salto, Cabreúva, Juquitiba and Valinhos. According to the National Association of Mushroom Producers (2013), there are more than 300 mushroom producers in the country, as the majority of them are micro- and small family farmers (National Association of Mushroom Producers, 2013; Zanatta, 2020).
Agaricus stands out. Bisporus (Paris champignon), Lentinula edodes (shiake) and Pleurotus (shimeji or hiratake) (National Association of Mushroom Producers, 2013). The cultivation of the shiitake mushroom began in Brazil in the early 1990s, and currently, its production has significantly increased because of
the viability of its cultivation in small areas, which currently occupies second place in production in relation to all mushrooms cultivated worldwide (Maciel, 2012).
Mushroom reproduction can occur sexually or asexually, with sexual reproduction carried out through spores and asexual reproduction carried out by the multiplication of any part of the fruiting body (Rosa, 2006).
Fungi play a major role in the ecosystem, as they have the ability to degrade agricultural production substrates, and these are the most significant microorganisms involved in the decomposition of organic raw material due to their characteristic degradative action. This occurs due to the production of extracellular enzymes, mainly lignocellulose, which is important for the degradation of substrate components (Válazquez-Cedeño; Mata; Savoie, 2002).
As a consequence of the relevant increase in the production and consumption of mushrooms, there has been an increase in the volume of waste generated, resulting from the harvesting and processing stages, which are composed mainly of the stem (the base of t he mushroom), which is generally discarded owing to its characteristics. unwanted sensory effects by consumers (Zhang et al., 2013), which represents 25–33% of the product's weight (Chou; Sheih; Fang, 2013). This results in volumes of 2.5 to 3.3 million tons of waste per year worldwide, which are discarded, representing an environmental problem, in addition to not generating economic gains for the producer or industry (Zhang et al. , 2013).
Shiitake mushrooms are known for being foods with high nutritional value, with high fiber content (41.92%), protein content (18.98%) and low lipid content (4.39%) (Furlani; Godoy, 2007). Furthermore, they present a series of bioactive properties, such as antifungal, antimicrobial (Hearst et al., 2009), and antitumor (Finimundy et al.). 2013) and antioxidant activity (Kitzberger et al., 2007). They are also known for being flavor enhancers, as they contain compounds that provide umami flavors (Poojary et al., 2017).
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Edible mushrooms, in turn, because they Considering the nutritional, functional
have these components that benefit the body, can contribute to reducing malnutrition or add to a good diet because of their high nutritional value (Moura, 2008).
Given the high nutritional and functional value of the mushroom, it is expected that its stem has a similar composition, which indicates that it is a potential material for studying its reuse as an ingredient in different foods, providing waste with an environmentally correct destination. This would make the activity even more sustainable, since in the production of mushrooms, the substrates used are mostly agricultural and agro - industrial residues, such as straw, sawdust, sugarcane bagasse, wheat bran and rice bran, which, after use, can also be used to fertilize vegetable gardens and plantations (Furlani; Godoy, 2007). Therefore, the development of alternative flours rich in proteins and fibers adds functional characteristics to these products and could be used in the preparation of bakery products, pasta and cereal bars (Sarinho; Cavalcanti; Oliveira, 2021).
and sensorial potential of the shiitake mushroom, studies on the chemical composition of its byproduct, the stipe, are important to characterize the material and develop future applications of this agro-industrial residue in the food industry, representing an economic alternative for producers and agro-industries, nutritional and health for consumers and sustainability for the environment. This work aims to develop a production protocol for shiitake mushroom stipe flour (Lentinula edodes) to reuse it and make the byproduct possible for human consumption.
2 MATERIALS AND METHODS
2.1 Material
The samples used to develop the work were shiitake mushroom stems donated in July 2020 by the company Fungibras, which is located in Botucatu–SP. To develop the shiitake stalk flour, the steps in the protocol in Figure 1 were followed.
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Figure 1. Shiitake stalk flour production protocol

Source: Prepared by the author (ABNT – Nbr 12607) (2024).
2.2 Methods
The collected samples were frozen via the slow freezing process (-18°C) and transported in thermal boxes to UNESP - Botucatu, where they were kept in a freezer at -18°C until use. The samples were thawed and sanitized in an aqueous solution of 200 ppm sodium hypochlorite for 10 minutes, followed by rinsing in good- quality drinking water. Then, the samples were cut (width of approximately 1 cm) and blanched with boiling water (100°C ± 1) for 2–3 minutes, followed by cooling with ice water (Maray;
Mostafa; El-Fakhrany, 2018) to prevent enzymatic browning promoted mainly by tyrosinase and catecholase enzymes (Moda et al ., 2005). Drying was carried out in an oven with air renewal at 70°C for 12 hours. All dehydrated material was packed in plastic packaging, stored in a dry, ventilated place and protected from light. Grinding was carried out in a Tecnal knife mill model TE-650/1, and subsequently, the byproducts were sieved (35 mesh). To calculate the yield of the stems after drying, Equation (1) was used.
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%Yield = (weight of wet vines – weight of dry vines) × 100
(1)
2.2.1 Determination of hydrogen potential (pH) pH readings were performed on a bench
pH meter equipped with a glass electrode (Micronal; model TEC-5) via the method described by the Association of Official Analytical Chemists (1995). A total of 10 g of shiitake mushroom stipe flour was weighed in a 250 ml beaker, 100 ml of distilled water was added, and the pH of the solution was measured. The analysis was performed in triplicate.
2.2.2 Determination of water activity
Water activity was determined in
The hue angle (hue angle) is the value in degrees corresponding to the three- dimensional color diagram, starting on the +a axis, with 0° (+a) corresponding to red, (+b) 90° corresponding to yellow, 180° (-a) corresponding to green and 270° (-b) corresponding to blue. Chroma is represented by C*, which defines the intensity of the color; the chroma value is 0 in the center and increases according to the distance from it (Minolta, 1998). The numerical values of a* and b* were converted into hue angles and chroma values (which are the variables that best represent the color of the shiitake mushroom stipe flour), according to Equations 2 and 3.
triplicate, with samples stabilized at 25°C, via the AquaLab 4TE analyzer. This analysis was
=
−1 ( )
(2)
performed in triplicate.
2.2.3 Instrumental color determination
To determine the instrumental color of
∗ = √(( ∗)2 +( ∗)2 )
3 RESULTS AND DISCUSSION
(3)
mushroom stipe flour, a portable colorimeter device (Minolta Chroma Meter, Model CR- 400) with the following parameters was used: L* (brightness), varying from 0 (black) to 100 (white); a* (red/green intensity); b* (red/green intensity) yellow/blue) from the CIELab system; and D65 illuminating source calibrated on standard white porcelain with Y=93.7, x=0.3160 and y=0.3323 (Minolta, 1998). Color determination was carried out in triplicate.
The results obtained from the dry moisture analysis of the shiitake mushroom stipe flour are shown in Table 1. The results obtained from the luminosity analysis (L*), hue angle (Hue), chroma, pH, and water activity (Aw) of the shiitake stem flour are shown in Table 2. The stem yield after drying was 11.9%.
Table 1. Moisture (%) of shiitake stipe flour Component
%
Humidity 5.62 ± 0.78
Mean ± standard deviation. n=3.
The result obtained from the humidity analysis was 5.62%. Since the initial sample used in this study was dried with mushroom stipe flour, the moisture content was expected to be low. According to the work of Cunha et al . (2011), the evaluation of the composition of shiitake mushrooms dried at 55°C revealed a
moisture content of 11.34% in the product obtained by the producer in Brasília and 11.13% in the mushroom from the producer in Mogi das Cruzes. In another work, Lira, Carvalho and Oliveira (2016) reported a value of 10.24% in shiitake mushrooms dried at 60°C for 24 hours. Therefore, we can observe that the results in the
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literature are above what was obtained in this work. The discrepancies in moisture content between samples may be related to the fact that this study evaluated only the mushroom stipe, whereas the literature studies used the entire mushroom.
Furthermore, there are variations in drying time and temperature between studies, which influence the drying intensity and, consequently, the final moisture content of the samples. According to Mattila et al. (2001), one
of the most important factors in regard to nutritional value is the moisture content, which influences the amount of dry matter, that is, the content of the other components of the material under study. The author also stated that other environmental factors can affect the amount of moisture in mushrooms, such as temperature, relative humidity and pH, which explains the difference between the results presented in this work and the literature on the subject.
Table 2. Luminosity (L*), hue angle (°), chroma, pH, and water activity (Aw) of shiitake stipe flour
Parameters Shiitake stem
L* 31.77 ± 0.36
° Hue 45th
Chroma 0.55 ± 0.01
pH 5.90 ± 0.06
Aw 0.44 ± 0.012
Mean ± standard deviation. n=3.
The results obtained for instrumental color show that the L* parameter, which refers to luminosity, was 31.77. A slightly greater value (41.74) was reported for commercial shiitake stem flour in a study by Lin et al. (2008). Lira (2017) reported a value of 77.27 for the whole shiitake mushroom; that is, the flour developed in the present work is darker than that developed by this author. This darkening possibly occurred because this was the study of the entire mushroom in the literature and because the time used to dry the Lira (2017) sample was longer (24 hours).
The hue angle was 45°, and according to Figure 2, the hue angle was orange, and the intensity was 0.55, as shown in Figure 3. Figure 4 shows a photo of the finished flour.
The color parameters are related to the part of the mushroom evaluated, as well as the
drying intensity, which can affect the color of the final product. In this sense, the application of mushroom stipe flour in products can result in different colors. traditional, which can lead the consumer to be influenced by color when purchasing.
The pH value obtained in this work was 5.90, which is close to that reported in the literature (6.12) (Lira; Carvalho; Oliveira, 2016). However, compared with data from Cunha et al . (2011), the pH of the shiitake mushroom sample produced in Brasília was 6.52, and that of the one produced in Mogi das Cruzes was 6.66. This variation in pH, in contrast to the results of this work, may be related to different factors, mainly the type of substrate used in cultivation, or may be related to the concentration of acids in the drying process and the part of the mushroom analyzed (stem) (Cunha et al., 2011).
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Figure 2. Color circle

Source: Nogueira et al. (2016).
Figure 3. L*C*h° color space
Source: Adapted from Ferreira and Spricigo ( 2017 ).

The water activity found in this work was 0.44. According to Lira, Carvalho and Oliveira (2016), the value obtained for shiitake flour was 0.54, and in another work, Cunha et al. (2011) reported a value of 0.23. These results indicate
that the products are microbiologically stable at room temperature, as they present a water activity lower than 0.6, which is considered limiting for the development of microorganisms (Lira; Carvalho; Oliveira, 2016).
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Figure 4. Photo of finished flour

Source: Prepared by the author (2024).
4 CONCLUSION
Under the conditions under which this work was carried out, it can be concluded that mushroom stipe flour can be recommended for use in food. It has a low moisture content (5.62%) and low water activity (0.44%), is microbiologically stable at room temperature and is slightly dark according to its light content (L*) (31.77).
5 REFERENCE
NATIONAL ASSOCIATION OF MUSHROOM PRODUCERS. Mushrooms. São José dos Pinhais: International Society for Mushroom Science , 2013 . Available at: https://www.anpc c ogumelos.org/cogumelos . Accessed on: 23 Dec. 2020.
ASSOCIATION OF OFFICIAL ANALYTICAL CHEMISTS. official Methods of Analysis of AOAC International . Washington, DC: Aoac Intl ., 1995.
CHOU, WT; SHEIH, IC; FANG, TJ The applications of polysaccharides from various mushroom wastes as prebiotics in different systems. Journal of Food Science , Champaign , vol. 78, no. 7, p. 1750-3841, 2013.
CUNHA, ALB; CAMPOS, CS; VAREJÃO, MJC; ARAÚJO, LM Nutritional value of edible mushrooms sold in Manaus-AM. In: JOURNAL OF SCIENTIFIC INITIATION PIBIC INP, 20., 2011, Manaus . Annals . [...]. Manaus: CNPq: FAPEAM, 2011. p. 1-5. Available at: https://repositorio.inpa.gov.br/bitstream/1/4173/ 1/pibic_inpa.pdf. Accessed on: 13 April. 2021.
FAOSTAT. Food and agriculture Date . São Paulo: Statistics Database , 2017. Available at: http://www.fao.org/faostat/en/#home. Accessed on: May 12th. 2021.
FAOSTAT. Food and agriculture Date . São Paulo: Statistics Database , 2024. Available at:
77 Nunes e Vieites/Protocolo de produção.../v38n4p69-79 (2023)
http://www.fao.org/faostat/en/#home. Accessed on: 20 June. 2024.
FERREIRA, MD; SPRICIGO, PC Colorimetry - principles and applications in agriculture. São Paulo: Embrapa Instrumentação, 2017.
FINIMUNDY, TC; GAMBATO, G.; FONTANA, R.; CAMASSOLA, M.; SALVADOR, M.; MOURA, S.; HESS, J.; HENRIQUES, JA; DILLON, AJ; ROESCH - ELY, M. Aqueous extracts of Lentinula edodes and Pleurotus sajor - cashew exhibit high antioxidant capability and promising in vitro antitumor activity . Nutrition Research , Tarrytown , vol. 33, no. 1, p. 76-84, 2013.
FLOUDAS, D.; RILEY, R.; BARRY, K.; BINDER, M. The Paleozoic Origin of Enzymatic Lignin Decomposition Reconstructed from 31 Fungal Genomes . Science , New York, vol. 336, no. 6089, p. 1715-1719, 29 June. 2012.
FURLANI, RPZ; GODOY, HT Nutritional value of edible mushrooms. Food Science and Technology , Campinas, v. 27, no. 1, p. 154 - 157, mar. 2007. Available at: https://www.scielo.br/j/cta/a/778cD6MTPJ5KfY Z6y7GyW8h/?format=pdf&lang=pt. Accessed on: 20 Mar. 2021.
HEARST, R; NELSON, D.; MCCOLLUM, G.; MILLAR, BC; MAEDA, Y.; GOLDSMITH, CE; ROONEY, P.J.; LOUGHREY, A.; RAO, JR; MOORE, J.E. An examination of antibacterial and antifungal properties of constituents of Shiitake (Lentinula edodes) and Oyster (Pleurotus ostreatus) mushrooms . Complementary Therapies In Clinical Practice , Lisbon, v. 15, no. 1, p. 5-7, Feb. 2009. DOI: http://dx.doi.org/10.1016/j.ctcp.2008.10.002. Available at: https://pubmed.ncbi.nlm.nih.gov/19161947/. Accessed on: 12 Oct. 2023.
KITZBERGER, CSG; SMÂNIA, A.; PEDROSA, RC; FERREIRA, SRS Antioxidant
and antimicrobial activities of shiitake (Lentinula edodes) extracts obtained by organic solvents and supercritical fluids . Journal Of Food Engineering , Essex, vol. 80, no. 2, p. 631-638, May 2007. DOI: http://dx.doi.org/10.1016/j.jfoodeng.2006.06.01 3. Available at: https://www.sciencedirect.com/science/article/a bs/pii/S0260877406004857. Accessed on: 15 September. 2023.
LIN, LY; TSENG, YH; LI, RC; MAU, JL Quality of shiitake stipe bread . Journal of Food Processing and Preservation , Westport , vol. 32, no. 6, p. 1002-1015, Dec. 2008. DOI: http://dx.doi.org/10.1111/j.1745 - 4549.2008.00229.x. Available at: https://www.researchgate.net/publication/22992 7461_Quality_of_shiitake_stipe_bread. Accessed on: 15 September. 2023.
LIRA, AP Application of mushroom flour in bread production and evaluation of technological and sensorial properties . 2017. Dissertation (Master’s in Food Engineering and Science) – State University of Southwest Bahia, Itapetinga, 2017.
LIRA, PA; CARVALHO, AS; OLIVEIRA, PC Obtaining and characterizing edible mushroom flours . In : BRAZILIAN CHEMISTRY CONGRESS, 56., 2016, Belém. Anais [...]. Rio de Janeiro: CNPq: FAPESPA , 2016. p. 1- 1. Available at: http://www.abq.org.br/cbq/2016/trabalhos/10/10 281-23416.html. Access in: 22 sea. 2021.
MACIEL, WP Lentinula Cultivation edones under different substrate and temperature conditions. 2012. Dissertation (Master’s in Agricultural Microbiology) – Federal University of Lavras, Lavras, 2012.
MARAY, ARM; MOSTAFA, MK; EL - FAKHRANY, AEDMA Effect of pretreatments and drying methods on physico-chemical , sensory characteristics and nutritional value of oyster mushroom . Journal of Food Processing
Nunes e Vieites/Protocolo de produção.../v38n4p69-79 (2023) 78
and Preservation , Westport , vol. 42, no. 1, p. 1-8, 9 May 2017.
MARTÍNEZ-IBARRA, E.; GÓMEZ- MARTÍN, MB; ARMESTO-LÓPEZ, XA Climatic and Socioeconomic Aspects of Mushrooms : the case of Spain. Sustainability , Barcelona, v. 11, no. 4, p. 1030, 16 Feb. 2019. DOI: http://dx.doi.org/10.3390/su11041030. Available at: https://www.mdpi.com/2071 - 1050/11/4/1030. Accessed on: 11 September. 2024. year.
MATTILA, P.; KÖNKÖ, K.; EUROLA, M.; PIHLAVA, J. M; ASTOLA, J.; VAHTERISTO, L.; HIETANIEMI, V.; KUMPULAINEN, J.; VALTONEN, M.; PIIRONEN, V. Contents of Vitamins , Mineral Elements , and Some Phenolic Compounds in Cultivated Mushrooms . Journal of Agricultural And Food Chemistry , Easton, vol. 49, no. 5, p. 2343-2348, apr. 2001. DOI: http://dx.doi.org/10.1021/jf001525d. Available at: https://pubs.acs.org/doi/pdf/10.1021/jf001525d. Accessed on: 12 Mar. 2023.
MINISTRY OF INDUSTRY, FOREIGN TRADE AND SERVICES. Mushrooms gain space on the plate and cultivation grows in Brazil . Brasília, DF: MDIC, 2023. Available at: https://www.gov.br/mdic/pt-br. Accessed on: 10 Aug. 2023.
MINOLTA, K. Precise color communication: quality control from perception to instrumentation. Osaka: AEBDPK®, 1998.
FASHION, EM ; SPOTO, MHF; HORII, J.; ZOCCHI, SS Use of hydrogen peroxide and citric acid in the conservation of Pleurotus mushrooms sajor - cashew in natura . Food Sciences and Technology , Campinas, v. 25, no. 2, p. 291-296, 2005.
MOURA, PLC Determination of essential and toxic elements in edible mushrooms by neutron activation analysis . 2008. Dissertation (Master of Science in the Area of Nuclear
Technology/Applications) – University of São Paulo, São Paulo, 2008.
NOGUEIRA, BA; VIEIRA IS. NR; PINTO, CLO; PINTO, CMF; RAMOS, AM Physicochemical characterization of chili pepper sauce (Capsicum frutensens L. ) processed according to good manufacturing practices. In: BRAZILIAN CONGRESS OF FOOD SCIENCE AND TECHNOLOGY, 25., 2016, Gramado. Annals [...]. Gramado: CNPq, 2016. p. 1- 5.
POOJARY, MM; ORLIEN, V.; PASSAMONTI, P.; OLSEN, K. Improved extraction methods for simultaneous recovery of umami compounds from six different mushrooms . Journal of Food Composition and Analysis , San Diego, vol. 63, p. 171- 183, 2017.
ROSA, LH Technical dossier . Cultivation of the “Sun Mushroom” . Minas Gerais: Cetec , 2006. Available at: http://www.respostatecnica.org.br/dossie - tecnico/downloadsDT/MzU=. Access in : 23 sea. 2021.
SARINHO, AMM; CAVALCANTI, MS; OLIVEIRA, IM Integral use of food: sustainability and use of modified flours. Recima 21 - Multidisciplinary Scientific Journal , São Paulo , v. 2, n. 10, p. 210-763, 18 nov. 2021. DOI: http://dx.doi.org/10.47820/recima21.v2i10.763. Available at: https://recima21.com.br/index.php/recima21/arti cle/view/763/715. Accessed on: 15 April. 2022.
URBEN, AF Mushroom production using modified Chinese technology : biotechnology and applications in agriculture and health. 3rd ed. Brasília, DF: Técnica, 2017.
VÁLAZQUEZ-CEDEÑO, MA; MATA, G.; SAVOIE, JM Waste reducing cultivation of pleurotus and pleurotus pulmonaryis on coffee pulp changes in the production of some lignocellulolytics enzymes . Word Journal of
79 Nunes e Vieites/Protocolo de produção.../v38n4p69-79 (2023)
Microbiology and Biotechnology , Netherlands , v. 8, no. 3, p. 201-207, Jan. 2002.
ZANATTA. Organic Cultivation of Shimeji and Hiratake . Santo Antônio de Posse:Zanatta , 2020. Available at: https://www.zanatta.com.br/cultivo-organico-de - shimeji/. Accessed on: 22 April. 2022.
ZHANG, N.; CHEN, H.; ZHANG, Y.; MA, L.; XU, X. Comparative studies on chemicals parameters and antioxidant properties of stipes and caps of shiitake mushroom as affected by different drying methods . Journal of the Science of Food and Agriculture , London, vol. 93, no. 12, p. 3107-3113, May 2013.